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MSCs modulated in SC hydrogels promote functional recovery in a murine hindlimb ischemia model. (a) Representative <t>LDPI</t> images of murine hindlimb ischemia model mice transplanted with PBS, hydrogel-only (Hydrogel), MSCs cultured on tissue culture plastic dishes (MSC), soft SC hydrogel encapsulating MSCs (Hydrogel + MSCs), and soft SC hydrogel encapsulating MSCs pretreated with PP2 (Hydrogel + MSCs + PP2) at 0, 3, 7, 14, 21, and 28 days post-operation. (b) Quantification of blood perfusion ratio from LDPI images. Data are presented as the mean ± SEM (n = 5). ∗∗ p < 0.01. (c) Representative photographs of ischemic limbs at day 28 post-operation. (d) Immunofluorescence staining of PCNA (red) in ischemic limbs at day 3 post-operation. Scale bar = 50 μm. (e) Quantification of the number of PCNA-positive cells. Data are presented as the mean ± SEM (n = 3). ∗∗ p < 0.01 vs. PBS, and ## p < 0.01 vs. Hydrogel + MSCs. (f) Immunofluorescence staining of cleaved caspase-3 (red) in ischemic limbs at day 3 post-operation. Scale bar = 50 μm. (g) Quantification of the number of cleaved caspase-3-positive cells. Data are presented as the mean ± SEM (n = 3). ∗ p < 0.05 and ∗∗ p < 0.01 vs. PBS, and ## p < 0.01 vs. Hydrogel + MSCs. (h) Immunofluorescence staining of F4/80 (green) in ischemic limbs at day 3 post-operation. Scale bar = 50 μm. (i) Quantification of the number of F4/80-positive cells. Data are presented as the mean ± SEM (n = 3). ∗∗ p < 0.01 vs. PBS, and # p < 0.05 and ## p < 0.01 vs. Hydrogel + MSCs. (j) Immunofluorescence staining of CD31 (green) in ischemic limbs at day 28 post-operation. Scale bar = 50 μm. (k) Quantification of the number of CD31-positive cells (capillary density) in ischemic tissues. Data are presented as the mean ± SEM (n = 3). ∗ p < 0.05 and ∗∗ p < 0.01 vs. PBS, and ## p < 0.01 vs. Hydrogel + MSCs. (l) Immunofluorescence staining of α-SMA (green) in ischemic limbs at day 28 post-operation. Scale bar = 50 μm. (m) Quantification of the number of α-SMA-positive cells (arteriole density) in ischemic tissues. Data are presented as the mean ± SEM (n = 3). ∗ p < 0.05 and ∗∗ p < 0.01 vs. PBS, and ## p < 0.01 vs. Hydrogel + MSCs.
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MSCs modulated in SC hydrogels promote functional recovery in a murine hindlimb ischemia model. (a) Representative <t>LDPI</t> images of murine hindlimb ischemia model mice transplanted with PBS, hydrogel-only (Hydrogel), MSCs cultured on tissue culture plastic dishes (MSC), soft SC hydrogel encapsulating MSCs (Hydrogel + MSCs), and soft SC hydrogel encapsulating MSCs pretreated with PP2 (Hydrogel + MSCs + PP2) at 0, 3, 7, 14, 21, and 28 days post-operation. (b) Quantification of blood perfusion ratio from LDPI images. Data are presented as the mean ± SEM (n = 5). ∗∗ p < 0.01. (c) Representative photographs of ischemic limbs at day 28 post-operation. (d) Immunofluorescence staining of PCNA (red) in ischemic limbs at day 3 post-operation. Scale bar = 50 μm. (e) Quantification of the number of PCNA-positive cells. Data are presented as the mean ± SEM (n = 3). ∗∗ p < 0.01 vs. PBS, and ## p < 0.01 vs. Hydrogel + MSCs. (f) Immunofluorescence staining of cleaved caspase-3 (red) in ischemic limbs at day 3 post-operation. Scale bar = 50 μm. (g) Quantification of the number of cleaved caspase-3-positive cells. Data are presented as the mean ± SEM (n = 3). ∗ p < 0.05 and ∗∗ p < 0.01 vs. PBS, and ## p < 0.01 vs. Hydrogel + MSCs. (h) Immunofluorescence staining of F4/80 (green) in ischemic limbs at day 3 post-operation. Scale bar = 50 μm. (i) Quantification of the number of F4/80-positive cells. Data are presented as the mean ± SEM (n = 3). ∗∗ p < 0.01 vs. PBS, and # p < 0.05 and ## p < 0.01 vs. Hydrogel + MSCs. (j) Immunofluorescence staining of CD31 (green) in ischemic limbs at day 28 post-operation. Scale bar = 50 μm. (k) Quantification of the number of CD31-positive cells (capillary density) in ischemic tissues. Data are presented as the mean ± SEM (n = 3). ∗ p < 0.05 and ∗∗ p < 0.01 vs. PBS, and ## p < 0.01 vs. Hydrogel + MSCs. (l) Immunofluorescence staining of α-SMA (green) in ischemic limbs at day 28 post-operation. Scale bar = 50 μm. (m) Quantification of the number of α-SMA-positive cells (arteriole density) in ischemic tissues. Data are presented as the mean ± SEM (n = 3). ∗ p < 0.05 and ∗∗ p < 0.01 vs. PBS, and ## p < 0.01 vs. Hydrogel + MSCs.
Laser Doppler Perfusion Imaging Periscan Pim 3, supplied by Perimed Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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periscan pim 3  (Perimed Inc)
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MSCs modulated in SC hydrogels promote functional recovery in a murine hindlimb ischemia model. (a) Representative <t>LDPI</t> images of murine hindlimb ischemia model mice transplanted with PBS, hydrogel-only (Hydrogel), MSCs cultured on tissue culture plastic dishes (MSC), soft SC hydrogel encapsulating MSCs (Hydrogel + MSCs), and soft SC hydrogel encapsulating MSCs pretreated with PP2 (Hydrogel + MSCs + PP2) at 0, 3, 7, 14, 21, and 28 days post-operation. (b) Quantification of blood perfusion ratio from LDPI images. Data are presented as the mean ± SEM (n = 5). ∗∗ p < 0.01. (c) Representative photographs of ischemic limbs at day 28 post-operation. (d) Immunofluorescence staining of PCNA (red) in ischemic limbs at day 3 post-operation. Scale bar = 50 μm. (e) Quantification of the number of PCNA-positive cells. Data are presented as the mean ± SEM (n = 3). ∗∗ p < 0.01 vs. PBS, and ## p < 0.01 vs. Hydrogel + MSCs. (f) Immunofluorescence staining of cleaved caspase-3 (red) in ischemic limbs at day 3 post-operation. Scale bar = 50 μm. (g) Quantification of the number of cleaved caspase-3-positive cells. Data are presented as the mean ± SEM (n = 3). ∗ p < 0.05 and ∗∗ p < 0.01 vs. PBS, and ## p < 0.01 vs. Hydrogel + MSCs. (h) Immunofluorescence staining of F4/80 (green) in ischemic limbs at day 3 post-operation. Scale bar = 50 μm. (i) Quantification of the number of F4/80-positive cells. Data are presented as the mean ± SEM (n = 3). ∗∗ p < 0.01 vs. PBS, and # p < 0.05 and ## p < 0.01 vs. Hydrogel + MSCs. (j) Immunofluorescence staining of CD31 (green) in ischemic limbs at day 28 post-operation. Scale bar = 50 μm. (k) Quantification of the number of CD31-positive cells (capillary density) in ischemic tissues. Data are presented as the mean ± SEM (n = 3). ∗ p < 0.05 and ∗∗ p < 0.01 vs. PBS, and ## p < 0.01 vs. Hydrogel + MSCs. (l) Immunofluorescence staining of α-SMA (green) in ischemic limbs at day 28 post-operation. Scale bar = 50 μm. (m) Quantification of the number of α-SMA-positive cells (arteriole density) in ischemic tissues. Data are presented as the mean ± SEM (n = 3). ∗ p < 0.05 and ∗∗ p < 0.01 vs. PBS, and ## p < 0.01 vs. Hydrogel + MSCs.
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MSCs modulated in SC hydrogels promote functional recovery in a murine hindlimb ischemia model. (a) Representative <t>LDPI</t> images of murine hindlimb ischemia model mice transplanted with PBS, hydrogel-only (Hydrogel), MSCs cultured on tissue culture plastic dishes (MSC), soft SC hydrogel encapsulating MSCs (Hydrogel + MSCs), and soft SC hydrogel encapsulating MSCs pretreated with PP2 (Hydrogel + MSCs + PP2) at 0, 3, 7, 14, 21, and 28 days post-operation. (b) Quantification of blood perfusion ratio from LDPI images. Data are presented as the mean ± SEM (n = 5). ∗∗ p < 0.01. (c) Representative photographs of ischemic limbs at day 28 post-operation. (d) Immunofluorescence staining of PCNA (red) in ischemic limbs at day 3 post-operation. Scale bar = 50 μm. (e) Quantification of the number of PCNA-positive cells. Data are presented as the mean ± SEM (n = 3). ∗∗ p < 0.01 vs. PBS, and ## p < 0.01 vs. Hydrogel + MSCs. (f) Immunofluorescence staining of cleaved caspase-3 (red) in ischemic limbs at day 3 post-operation. Scale bar = 50 μm. (g) Quantification of the number of cleaved caspase-3-positive cells. Data are presented as the mean ± SEM (n = 3). ∗ p < 0.05 and ∗∗ p < 0.01 vs. PBS, and ## p < 0.01 vs. Hydrogel + MSCs. (h) Immunofluorescence staining of F4/80 (green) in ischemic limbs at day 3 post-operation. Scale bar = 50 μm. (i) Quantification of the number of F4/80-positive cells. Data are presented as the mean ± SEM (n = 3). ∗∗ p < 0.01 vs. PBS, and # p < 0.05 and ## p < 0.01 vs. Hydrogel + MSCs. (j) Immunofluorescence staining of CD31 (green) in ischemic limbs at day 28 post-operation. Scale bar = 50 μm. (k) Quantification of the number of CD31-positive cells (capillary density) in ischemic tissues. Data are presented as the mean ± SEM (n = 3). ∗ p < 0.05 and ∗∗ p < 0.01 vs. PBS, and ## p < 0.01 vs. Hydrogel + MSCs. (l) Immunofluorescence staining of α-SMA (green) in ischemic limbs at day 28 post-operation. Scale bar = 50 μm. (m) Quantification of the number of α-SMA-positive cells (arteriole density) in ischemic tissues. Data are presented as the mean ± SEM (n = 3). ∗ p < 0.05 and ∗∗ p < 0.01 vs. PBS, and ## p < 0.01 vs. Hydrogel + MSCs.
Pim 3 Laser Doppler Perfusion Imager, supplied by Perimed Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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laser doppler perfusion imaging system periscan pim 3  (Perimed Inc)
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MSCs modulated in SC hydrogels promote functional recovery in a murine hindlimb ischemia model. (a) Representative <t>LDPI</t> images of murine hindlimb ischemia model mice transplanted with PBS, hydrogel-only (Hydrogel), MSCs cultured on tissue culture plastic dishes (MSC), soft SC hydrogel encapsulating MSCs (Hydrogel + MSCs), and soft SC hydrogel encapsulating MSCs pretreated with PP2 (Hydrogel + MSCs + PP2) at 0, 3, 7, 14, 21, and 28 days post-operation. (b) Quantification of blood perfusion ratio from LDPI images. Data are presented as the mean ± SEM (n = 5). ∗∗ p < 0.01. (c) Representative photographs of ischemic limbs at day 28 post-operation. (d) Immunofluorescence staining of PCNA (red) in ischemic limbs at day 3 post-operation. Scale bar = 50 μm. (e) Quantification of the number of PCNA-positive cells. Data are presented as the mean ± SEM (n = 3). ∗∗ p < 0.01 vs. PBS, and ## p < 0.01 vs. Hydrogel + MSCs. (f) Immunofluorescence staining of cleaved caspase-3 (red) in ischemic limbs at day 3 post-operation. Scale bar = 50 μm. (g) Quantification of the number of cleaved caspase-3-positive cells. Data are presented as the mean ± SEM (n = 3). ∗ p < 0.05 and ∗∗ p < 0.01 vs. PBS, and ## p < 0.01 vs. Hydrogel + MSCs. (h) Immunofluorescence staining of F4/80 (green) in ischemic limbs at day 3 post-operation. Scale bar = 50 μm. (i) Quantification of the number of F4/80-positive cells. Data are presented as the mean ± SEM (n = 3). ∗∗ p < 0.01 vs. PBS, and # p < 0.05 and ## p < 0.01 vs. Hydrogel + MSCs. (j) Immunofluorescence staining of CD31 (green) in ischemic limbs at day 28 post-operation. Scale bar = 50 μm. (k) Quantification of the number of CD31-positive cells (capillary density) in ischemic tissues. Data are presented as the mean ± SEM (n = 3). ∗ p < 0.05 and ∗∗ p < 0.01 vs. PBS, and ## p < 0.01 vs. Hydrogel + MSCs. (l) Immunofluorescence staining of α-SMA (green) in ischemic limbs at day 28 post-operation. Scale bar = 50 μm. (m) Quantification of the number of α-SMA-positive cells (arteriole density) in ischemic tissues. Data are presented as the mean ± SEM (n = 3). ∗ p < 0.05 and ∗∗ p < 0.01 vs. PBS, and ## p < 0.01 vs. Hydrogel + MSCs.
Laser Doppler Perfusion Imaging System Periscan Pim 3, supplied by Perimed Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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MSCs modulated in SC hydrogels promote functional recovery in a murine hindlimb ischemia model. (a) Representative <t>LDPI</t> images of murine hindlimb ischemia model mice transplanted with PBS, hydrogel-only (Hydrogel), MSCs cultured on tissue culture plastic dishes (MSC), soft SC hydrogel encapsulating MSCs (Hydrogel + MSCs), and soft SC hydrogel encapsulating MSCs pretreated with PP2 (Hydrogel + MSCs + PP2) at 0, 3, 7, 14, 21, and 28 days post-operation. (b) Quantification of blood perfusion ratio from LDPI images. Data are presented as the mean ± SEM (n = 5). ∗∗ p < 0.01. (c) Representative photographs of ischemic limbs at day 28 post-operation. (d) Immunofluorescence staining of PCNA (red) in ischemic limbs at day 3 post-operation. Scale bar = 50 μm. (e) Quantification of the number of PCNA-positive cells. Data are presented as the mean ± SEM (n = 3). ∗∗ p < 0.01 vs. PBS, and ## p < 0.01 vs. Hydrogel + MSCs. (f) Immunofluorescence staining of cleaved caspase-3 (red) in ischemic limbs at day 3 post-operation. Scale bar = 50 μm. (g) Quantification of the number of cleaved caspase-3-positive cells. Data are presented as the mean ± SEM (n = 3). ∗ p < 0.05 and ∗∗ p < 0.01 vs. PBS, and ## p < 0.01 vs. Hydrogel + MSCs. (h) Immunofluorescence staining of F4/80 (green) in ischemic limbs at day 3 post-operation. Scale bar = 50 μm. (i) Quantification of the number of F4/80-positive cells. Data are presented as the mean ± SEM (n = 3). ∗∗ p < 0.01 vs. PBS, and # p < 0.05 and ## p < 0.01 vs. Hydrogel + MSCs. (j) Immunofluorescence staining of CD31 (green) in ischemic limbs at day 28 post-operation. Scale bar = 50 μm. (k) Quantification of the number of CD31-positive cells (capillary density) in ischemic tissues. Data are presented as the mean ± SEM (n = 3). ∗ p < 0.05 and ∗∗ p < 0.01 vs. PBS, and ## p < 0.01 vs. Hydrogel + MSCs. (l) Immunofluorescence staining of α-SMA (green) in ischemic limbs at day 28 post-operation. Scale bar = 50 μm. (m) Quantification of the number of α-SMA-positive cells (arteriole density) in ischemic tissues. Data are presented as the mean ± SEM (n = 3). ∗ p < 0.05 and ∗∗ p < 0.01 vs. PBS, and ## p < 0.01 vs. Hydrogel + MSCs.
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MSCs modulated in SC hydrogels promote functional recovery in a murine hindlimb ischemia model. (a) Representative <t>LDPI</t> images of murine hindlimb ischemia model mice transplanted with PBS, hydrogel-only (Hydrogel), MSCs cultured on tissue culture plastic dishes (MSC), soft SC hydrogel encapsulating MSCs (Hydrogel + MSCs), and soft SC hydrogel encapsulating MSCs pretreated with PP2 (Hydrogel + MSCs + PP2) at 0, 3, 7, 14, 21, and 28 days post-operation. (b) Quantification of blood perfusion ratio from LDPI images. Data are presented as the mean ± SEM (n = 5). ∗∗ p < 0.01. (c) Representative photographs of ischemic limbs at day 28 post-operation. (d) Immunofluorescence staining of PCNA (red) in ischemic limbs at day 3 post-operation. Scale bar = 50 μm. (e) Quantification of the number of PCNA-positive cells. Data are presented as the mean ± SEM (n = 3). ∗∗ p < 0.01 vs. PBS, and ## p < 0.01 vs. Hydrogel + MSCs. (f) Immunofluorescence staining of cleaved caspase-3 (red) in ischemic limbs at day 3 post-operation. Scale bar = 50 μm. (g) Quantification of the number of cleaved caspase-3-positive cells. Data are presented as the mean ± SEM (n = 3). ∗ p < 0.05 and ∗∗ p < 0.01 vs. PBS, and ## p < 0.01 vs. Hydrogel + MSCs. (h) Immunofluorescence staining of F4/80 (green) in ischemic limbs at day 3 post-operation. Scale bar = 50 μm. (i) Quantification of the number of F4/80-positive cells. Data are presented as the mean ± SEM (n = 3). ∗∗ p < 0.01 vs. PBS, and # p < 0.05 and ## p < 0.01 vs. Hydrogel + MSCs. (j) Immunofluorescence staining of CD31 (green) in ischemic limbs at day 28 post-operation. Scale bar = 50 μm. (k) Quantification of the number of CD31-positive cells (capillary density) in ischemic tissues. Data are presented as the mean ± SEM (n = 3). ∗ p < 0.05 and ∗∗ p < 0.01 vs. PBS, and ## p < 0.01 vs. Hydrogel + MSCs. (l) Immunofluorescence staining of α-SMA (green) in ischemic limbs at day 28 post-operation. Scale bar = 50 μm. (m) Quantification of the number of α-SMA-positive cells (arteriole density) in ischemic tissues. Data are presented as the mean ± SEM (n = 3). ∗ p < 0.05 and ∗∗ p < 0.01 vs. PBS, and ## p < 0.01 vs. Hydrogel + MSCs.
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MSCs modulated in SC hydrogels promote functional recovery in a murine hindlimb ischemia model. (a) Representative LDPI images of murine hindlimb ischemia model mice transplanted with PBS, hydrogel-only (Hydrogel), MSCs cultured on tissue culture plastic dishes (MSC), soft SC hydrogel encapsulating MSCs (Hydrogel + MSCs), and soft SC hydrogel encapsulating MSCs pretreated with PP2 (Hydrogel + MSCs + PP2) at 0, 3, 7, 14, 21, and 28 days post-operation. (b) Quantification of blood perfusion ratio from LDPI images. Data are presented as the mean ± SEM (n = 5). ∗∗ p < 0.01. (c) Representative photographs of ischemic limbs at day 28 post-operation. (d) Immunofluorescence staining of PCNA (red) in ischemic limbs at day 3 post-operation. Scale bar = 50 μm. (e) Quantification of the number of PCNA-positive cells. Data are presented as the mean ± SEM (n = 3). ∗∗ p < 0.01 vs. PBS, and ## p < 0.01 vs. Hydrogel + MSCs. (f) Immunofluorescence staining of cleaved caspase-3 (red) in ischemic limbs at day 3 post-operation. Scale bar = 50 μm. (g) Quantification of the number of cleaved caspase-3-positive cells. Data are presented as the mean ± SEM (n = 3). ∗ p < 0.05 and ∗∗ p < 0.01 vs. PBS, and ## p < 0.01 vs. Hydrogel + MSCs. (h) Immunofluorescence staining of F4/80 (green) in ischemic limbs at day 3 post-operation. Scale bar = 50 μm. (i) Quantification of the number of F4/80-positive cells. Data are presented as the mean ± SEM (n = 3). ∗∗ p < 0.01 vs. PBS, and # p < 0.05 and ## p < 0.01 vs. Hydrogel + MSCs. (j) Immunofluorescence staining of CD31 (green) in ischemic limbs at day 28 post-operation. Scale bar = 50 μm. (k) Quantification of the number of CD31-positive cells (capillary density) in ischemic tissues. Data are presented as the mean ± SEM (n = 3). ∗ p < 0.05 and ∗∗ p < 0.01 vs. PBS, and ## p < 0.01 vs. Hydrogel + MSCs. (l) Immunofluorescence staining of α-SMA (green) in ischemic limbs at day 28 post-operation. Scale bar = 50 μm. (m) Quantification of the number of α-SMA-positive cells (arteriole density) in ischemic tissues. Data are presented as the mean ± SEM (n = 3). ∗ p < 0.05 and ∗∗ p < 0.01 vs. PBS, and ## p < 0.01 vs. Hydrogel + MSCs.

Journal: Bioactive Materials

Article Title: Mechanosignaling and 3D morphological adaptation of MSCs in response to hydrogel rigidity underpin angiogenic and immunomodulatory efficacy for ischemic injury regeneration

doi: 10.1016/j.bioactmat.2025.07.027

Figure Lengend Snippet: MSCs modulated in SC hydrogels promote functional recovery in a murine hindlimb ischemia model. (a) Representative LDPI images of murine hindlimb ischemia model mice transplanted with PBS, hydrogel-only (Hydrogel), MSCs cultured on tissue culture plastic dishes (MSC), soft SC hydrogel encapsulating MSCs (Hydrogel + MSCs), and soft SC hydrogel encapsulating MSCs pretreated with PP2 (Hydrogel + MSCs + PP2) at 0, 3, 7, 14, 21, and 28 days post-operation. (b) Quantification of blood perfusion ratio from LDPI images. Data are presented as the mean ± SEM (n = 5). ∗∗ p < 0.01. (c) Representative photographs of ischemic limbs at day 28 post-operation. (d) Immunofluorescence staining of PCNA (red) in ischemic limbs at day 3 post-operation. Scale bar = 50 μm. (e) Quantification of the number of PCNA-positive cells. Data are presented as the mean ± SEM (n = 3). ∗∗ p < 0.01 vs. PBS, and ## p < 0.01 vs. Hydrogel + MSCs. (f) Immunofluorescence staining of cleaved caspase-3 (red) in ischemic limbs at day 3 post-operation. Scale bar = 50 μm. (g) Quantification of the number of cleaved caspase-3-positive cells. Data are presented as the mean ± SEM (n = 3). ∗ p < 0.05 and ∗∗ p < 0.01 vs. PBS, and ## p < 0.01 vs. Hydrogel + MSCs. (h) Immunofluorescence staining of F4/80 (green) in ischemic limbs at day 3 post-operation. Scale bar = 50 μm. (i) Quantification of the number of F4/80-positive cells. Data are presented as the mean ± SEM (n = 3). ∗∗ p < 0.01 vs. PBS, and # p < 0.05 and ## p < 0.01 vs. Hydrogel + MSCs. (j) Immunofluorescence staining of CD31 (green) in ischemic limbs at day 28 post-operation. Scale bar = 50 μm. (k) Quantification of the number of CD31-positive cells (capillary density) in ischemic tissues. Data are presented as the mean ± SEM (n = 3). ∗ p < 0.05 and ∗∗ p < 0.01 vs. PBS, and ## p < 0.01 vs. Hydrogel + MSCs. (l) Immunofluorescence staining of α-SMA (green) in ischemic limbs at day 28 post-operation. Scale bar = 50 μm. (m) Quantification of the number of α-SMA-positive cells (arteriole density) in ischemic tissues. Data are presented as the mean ± SEM (n = 3). ∗ p < 0.05 and ∗∗ p < 0.01 vs. PBS, and ## p < 0.01 vs. Hydrogel + MSCs.

Article Snippet: Hindlimb blood flow was accessed using and LDPI PeriScan PIM 3 (Perimed, Järfälla, Sweden) by analyzing the ratio of blood flow in the ischemic limb (left) to the non-ligated limb (right) limb at 0, 3, 7, 14, 21, and 28 days of post-operation using PIMSoft (Perimed).

Techniques: Functional Assay, Cell Culture, Immunofluorescence, Staining